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KMID : 0613820190290060679
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Journal of Life Science
2019 Volume.29 No. 6 p.679 ~ p.687
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Anti-oxidative and Anti-cancer Activities of Ethanol Extract of Litsea populifolia
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Jin Soo-Jung
Oh You-Na
Jeong Hyun-Young
Yun Hee-Jung
Park Jung-Ha
Kwon Hyun-Ju
Kim Byung-Woo
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Abstract
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Litsea populifolia, a plant species of the Lauraceae family, is widely distributed in the tropical and subtropical areas of Asia. The phylogenetic relationships and botanical characteristics of L. populifolia have been reported; however, its anti-oxidative and anti-cancer activities remain unclear. In this study, we evaluated the anti-oxidative and anti-cancer effects of ethanol extracts of L. populifolia (EELP) together with the molecular mechanism of its anti-cancer activity in human lung adenocarcinoma A549 cells. EELP showed significant anti-oxidative effects with a 50% inhibitory concentration at 11.71 μg/ml, which was measured by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. EELP exhibited cytotoxic activity and induced cell cycle arrest at the G1 phase in A549 cells in a dose-dependent manner, whereas EELP did not have the cytotoxic effect on the normal human lung cell line IMR90. Treatment with EELP also resulted in a decreased expression of G1/S transition-related molecules?including cyclin-dependent kinase (CDK) 2, CDK6, cyclin D1, and cyclin E?both for the transcription and translation levels. EELP-induced G1 arrest was associated with the phosphorylation of checkpoint kinase 2 (CHK2), p53, cell division cycle 25 homolog A (CDC25A), and the reduction of CDC25A expression in A549 cells. Collectively, these results suggest that EELP may exert an anti-cancer effect by cell cycle arrest at the G1 phase through both p53-dependent and p53-independent (ATM/CHK2/ CDC25A/CDK2) pathways in A549 cells.
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KEYWORD
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A549 cells, anti-cancer, anti-oxidative, G1 arrest, Litsea populifolia
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